Micro Tracker

Micro Tracker is a method we established for visualizing results from different analysis methods in a traditional histopathological specimen.

Overview

We would like to be able to directly correlate results from methods such as Nucleotyping and DNA ploidy to histopathological classification and grading a comparative cell-by-cell analysis.

Background

The method is developed and implemented into RUST. Histological sections stained with standard Hematoxilin-Eosin (HE) are scanned in the NanoZoomer before they are de-stained and re-stained with Feulgen-Schiff and scanned with high resolution on Z1 (image cytometry system). The individual nuclei are then segmented and analyzed, before each nuclei is retrieved in the original HE-scan aided by Micro Tracker. The result from the nuclei measurements are then classified and defined groups can be chosen and given a clear markation (with different colors, for example) which are drawn around the same nuclei in the original HE-scan. The method also works on parallel sections.

Media